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goat anti mouse igg  (Bio-Rad)


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    Structured Review

    Bio-Rad goat anti mouse igg
    Goat Anti Mouse Igg, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 3744 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mouse+igg/Goat+anti+Mouse+IgG/pmc13004575-85-30-34
    Average 96 stars, based on 3744 article reviews
    goat anti mouse igg - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Membrane:

    Article Title: Extracellular vesicle-bound bacterial toxin pneumolysin triggers membrane engagement and damage beyond canonical pore formation
    Article Snippet: 30 μg of total EV protein was denatured using 1x Laemmli buffer (Biorad) containing 50 mM DTT and boiled at 70°C for 10 minutes and separated using 12-15% SDS polyacrylamide gel electrophoresis and transferred onto PVDF membrane. .. The membrane was then blocked with 5% BSA at room temperature for 1 h and and probed with mouse anti-CD81 (1:1000), rabbit anti-CD63 (1:1000), rabbit anti-Annexin A1 (1:1000) and mouse anti-PLY (1:500) incubated overnight at 4°C, followed by HRP-conjugated secondary antibody HRP-conjugated anti-mouse IgG and anti-rabbit IgG (1:3000, Biorad). .. The chemiluminescence was visualised with an iBright FL1500 Imaging System (Invitrogen).

    Incubation:

    Article Title: Extracellular vesicle-bound bacterial toxin pneumolysin triggers membrane engagement and damage beyond canonical pore formation
    Article Snippet: 30 μg of total EV protein was denatured using 1x Laemmli buffer (Biorad) containing 50 mM DTT and boiled at 70°C for 10 minutes and separated using 12-15% SDS polyacrylamide gel electrophoresis and transferred onto PVDF membrane. .. The membrane was then blocked with 5% BSA at room temperature for 1 h and and probed with mouse anti-CD81 (1:1000), rabbit anti-CD63 (1:1000), rabbit anti-Annexin A1 (1:1000) and mouse anti-PLY (1:500) incubated overnight at 4°C, followed by HRP-conjugated secondary antibody HRP-conjugated anti-mouse IgG and anti-rabbit IgG (1:3000, Biorad). .. The chemiluminescence was visualised with an iBright FL1500 Imaging System (Invitrogen).

    Article Title: Average local nucleosome motion remains constant during interphase in living human cells
    Article Snippet: The membranes were blocked with 10% or 3% skim milk (190-12865; Fujifilm Wako) for detection of H2B or HaloTag, respectively. .. The membranes were then incubated with primary antibodies: rabbit anti-H2B (1:10,000; ab1790, Abcam) or mouse anti-HaloTag (1:1,000; G9211, Promega), followed by the corresponding goat horseradish peroxidase (HRP)-conjugated secondary antibodies: anti-rabbit IgG (1:5000; 170-6515, Bio-Rad) or anti-mouse IgG (1:5000; 170-6516, Bio-Rad). .. Bands were detected by chemiluminescence reactions (WBKLS0100; Millipore), and images were acquired with the EZ-Capture MG (AE-9300H-CSP; ATTO).

    Article Title: Hepatitis C Virus Core Induces p53 Ser-15 Phosphorylation to Facilitate E6-Associated Protein-Mediated Proteasomal Degradation of p53
    Article Snippet: After blocking, membranes were incubated with primary antibodies, including total p53 (Santa Cruz Biotechnology #sc-126, 1:1000), pSer-15 p53 (Cell Signaling Technology #9284, 1:1000), pSer-20 p53 (Cell Signaling Technology #9285, 1:1000), E6AP (Thermo Fisher Scientific #PA3-843, 1:2000), MDM2 (Santa Cruz Biotechnology #sc-965, 1:1000), pSer-1981 ATM (Abcam #ab5883, 1:1000), pThr-68 Chk2 (Abcam #ab2661, 1:1000), c-Myc (Santa Cruz Biotechnology #sc-40, 1:500), HCV Core (Abcam #ab58713, 1:500), HA (Santa Cruz Biotechnology #sc-7392, 1:500), and γ-tubulin (Santa Cruz Biotechnology #sc-17787, 1:500). .. After binding with primary antibodies, membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies, including anti-mouse IgG (Bio-Rad #BR170-6516, 1:3000, Hercules, CA, USA), anti-rabbit IgG (Bio-Rad #BR170-6515, 1:3000), and anti-goat IgG (Thermo Fisher Scientific #31400, 1:10,000), for 1 h. Protein bands were visualized using an enhanced chemiluminescence kit (Advansta #K-12043-D20) and imaged on a ChemiDoc XRS system (Bio-Rad). ..

    Article Title: Hepatitis C Virus Core Induces p53 Ser-15 Phosphorylation to Facilitate E6-Associated Protein-Mediated Proteasomal Degradation of p53
    Article Snippet: After blocking, membranes were incubated with primary antibodies, including total p53 (Santa Cruz Biotechnology #sc-126, 1:1000), pSer-15 p53 (Cell Signaling Technology #9284, 1:1000), pSer-20 p53 (Cell Signaling Technology #9285, 1:1000), E6AP (Thermo Fisher Scientific #PA3-843, 1:2000), MDM2 (Santa Cruz Biotechnology #sc-965, 1:1000), pSer-1981 ATM (Abcam #ab5883, 1:1000), pThr-68 Chk2 (Abcam #ab2661, 1:1000), c-Myc (Santa Cruz Biotechnology #sc-40, 1:500), HCV Core (Abcam #ab58713, 1:500), HA (Santa Cruz Biotechnology #sc-7392, 1:500), and γ-tubulin (Santa Cruz Biotechnology #sc-17787, 1:500). .. After binding with primary antibodies, membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies, including anti-mouse IgG (Bio-Rad #BR170-6516, 1:3000, Hercules, CA, USA), anti-rabbit IgG (Bio-Rad #BR170-6515, 1:3000), and anti-goat IgG (Thermo Fisher Scientific #31400, 1:10,000), for 1 h. Protein bands were visualized using an enhanced chemiluminescence kit (Advansta #K-12043-D20) and imaged on a ChemiDoc XRS system (Bio-Rad). ..

    other:

    Article Title: A cancer-specific pipeline uncovers MCM3 as a driver of glioblastoma progression via suppression of the Wnt pathway.
    Article Snippet: Glioblastoma (GBM) is a deadly brain cancer with a poor prognosis.. Here, we developed DepMeta, a specialized pipeline designed to identify essential genes with stable prognostic value across various cancer types.. Applying DepMeta in conjunction with Weighted Gene Co-expression Network Analysis (WGCNA) to GBM resulted in the establishment of the DepMetaPath signature, a set of 12 genes critical for GBM progression.

    Cell-Signaling:

    Article Title: PARPi Combining Nanoparticle LIN28B siRNA for the Management of Malignant Ascites
    Article Snippet: .. The following antibodies were used: anti‐human LIN28B (Cell Signaling Technology, 4196S, 1:1000), anti‐mouse Lin28b (Proteintech, 11724‐1‐AP, 1:500), anti‐β‐actin (ABclonal, AC026, 1:10000), StarBright Blue 520 goat anti‐mouse IgG (Bio‐Rad, 12005867, 1:10000), Alexa Fluor Plus 800 goat anti‐rabbit IgG (H + L) (Invitrogen, A32735, 1:10000), Anti‐Mouse CD8a‐Purified In vivo (C375‐25 mg), Anti‐Mouse NK1.1‐Purified In vivo (N123‐25 mg), Human/Primate IL‐6 Mab(Clone 6708, MAB206‐SP, R&D), Human TNF‐alpha Mab(Clone28401, MAB610‐SP, R&D), anti‐CD31 antibody (Thermo Fisher Scientific, MA3105, 1:200), and anti‐TER‐119 antibody (BD Biosciences, 557915, 1:100). .. 2, 2′‐Thiobis(ethylamine) (Jiangsu Aikang Biomedical R&D Co., Ltd., catalog number: 871‐76‐1), acryloyl chloride (Tianjin Xiensi Biochemical Technology Co., Ltd., catalog number: 814‐68‐6), spermine (Tianjin Xiensi Biochemical Technology Co., Ltd., catalog number: 71‐44‐3), N,N'‐Methylenebisacrylamide (Tianjin Xiensi Biochemical Technology Co., Ltd., catalog number: 110‐26‐9), N,N‐Dimethylethylenediamine (Tianjin Xiensi Biochemical Technology Co., Ltd., catalog number: 108‐00‐9), cystamine dihydrochloride (Tianjin Xiensi Biochemical Technology Co., Ltd., catalog number: 56‐17‐7), folate‐conjugated polyethylene glycol phospholipid (DSPE‐PEG‐FA) (Aladdin, catalog number: B2224303), DMG‐Hy‐PEG3400 (purchased from Ruixi Biological, catalog number: R‐S0011), dioleoylphosphatidylethanolamine (DOPE) (Ruixi Biological, catalog number: 4004‐05‐1), distearoylphosphatidylethanolamine (DSPE) (Ruixi Biological, catalog number: 1069‐79‐0), and cholesterol (Tian'jin Xiensi Biochemical Technology Co., Ltd., catalog number: 57‐88‐5), Recombinant Human IL‐6(HEK293‐expressed) Protein (7270‐1L‐025, R&D), Clodronate Liposomes(From Vrije Universiteit Amsterdam, 40337ES10, Yeasen), Mouse anti‐PEG (Polyethylene glycol) IgG ELISA kit (EM2149, Fine Test, Wuhan Fine Biotech Co., Ltd.), Mouse bFGF ELISA Kit (Solarbio, SEKM‐0081), Mouse VEGF‐A ELISA Kit (bioswamp, MU30660),Mouse IL‐6 ELISA Kit (Multisciences, EK206), Mouse IL‐β ELISA Kit (Multisciences, 410 EK201B), Mouse IL‐1α ELISA Kit (Multisciences, EK201B), Mouse IL‐10 ELISA Kit (Multisciences, EK210), and Mouse GM‐CSF ELISA Kit (Multisciences, EK263).

    In Vivo:

    Article Title: PARPi Combining Nanoparticle LIN28B siRNA for the Management of Malignant Ascites
    Article Snippet: .. The following antibodies were used: anti‐human LIN28B (Cell Signaling Technology, 4196S, 1:1000), anti‐mouse Lin28b (Proteintech, 11724‐1‐AP, 1:500), anti‐β‐actin (ABclonal, AC026, 1:10000), StarBright Blue 520 goat anti‐mouse IgG (Bio‐Rad, 12005867, 1:10000), Alexa Fluor Plus 800 goat anti‐rabbit IgG (H + L) (Invitrogen, A32735, 1:10000), Anti‐Mouse CD8a‐Purified In vivo (C375‐25 mg), Anti‐Mouse NK1.1‐Purified In vivo (N123‐25 mg), Human/Primate IL‐6 Mab(Clone 6708, MAB206‐SP, R&D), Human TNF‐alpha Mab(Clone28401, MAB610‐SP, R&D), anti‐CD31 antibody (Thermo Fisher Scientific, MA3105, 1:200), and anti‐TER‐119 antibody (BD Biosciences, 557915, 1:100). .. 2, 2′‐Thiobis(ethylamine) (Jiangsu Aikang Biomedical R&D Co., Ltd., catalog number: 871‐76‐1), acryloyl chloride (Tianjin Xiensi Biochemical Technology Co., Ltd., catalog number: 814‐68‐6), spermine (Tianjin Xiensi Biochemical Technology Co., Ltd., catalog number: 71‐44‐3), N,N'‐Methylenebisacrylamide (Tianjin Xiensi Biochemical Technology Co., Ltd., catalog number: 110‐26‐9), N,N‐Dimethylethylenediamine (Tianjin Xiensi Biochemical Technology Co., Ltd., catalog number: 108‐00‐9), cystamine dihydrochloride (Tianjin Xiensi Biochemical Technology Co., Ltd., catalog number: 56‐17‐7), folate‐conjugated polyethylene glycol phospholipid (DSPE‐PEG‐FA) (Aladdin, catalog number: B2224303), DMG‐Hy‐PEG3400 (purchased from Ruixi Biological, catalog number: R‐S0011), dioleoylphosphatidylethanolamine (DOPE) (Ruixi Biological, catalog number: 4004‐05‐1), distearoylphosphatidylethanolamine (DSPE) (Ruixi Biological, catalog number: 1069‐79‐0), and cholesterol (Tian'jin Xiensi Biochemical Technology Co., Ltd., catalog number: 57‐88‐5), Recombinant Human IL‐6(HEK293‐expressed) Protein (7270‐1L‐025, R&D), Clodronate Liposomes(From Vrije Universiteit Amsterdam, 40337ES10, Yeasen), Mouse anti‐PEG (Polyethylene glycol) IgG ELISA kit (EM2149, Fine Test, Wuhan Fine Biotech Co., Ltd.), Mouse bFGF ELISA Kit (Solarbio, SEKM‐0081), Mouse VEGF‐A ELISA Kit (bioswamp, MU30660),Mouse IL‐6 ELISA Kit (Multisciences, EK206), Mouse IL‐β ELISA Kit (Multisciences, 410 EK201B), Mouse IL‐1α ELISA Kit (Multisciences, EK201B), Mouse IL‐10 ELISA Kit (Multisciences, EK210), and Mouse GM‐CSF ELISA Kit (Multisciences, EK263).

    Binding Assay:

    Article Title: Hepatitis C Virus Core Induces p53 Ser-15 Phosphorylation to Facilitate E6-Associated Protein-Mediated Proteasomal Degradation of p53
    Article Snippet: After blocking, membranes were incubated with primary antibodies, including total p53 (Santa Cruz Biotechnology #sc-126, 1:1000), pSer-15 p53 (Cell Signaling Technology #9284, 1:1000), pSer-20 p53 (Cell Signaling Technology #9285, 1:1000), E6AP (Thermo Fisher Scientific #PA3-843, 1:2000), MDM2 (Santa Cruz Biotechnology #sc-965, 1:1000), pSer-1981 ATM (Abcam #ab5883, 1:1000), pThr-68 Chk2 (Abcam #ab2661, 1:1000), c-Myc (Santa Cruz Biotechnology #sc-40, 1:500), HCV Core (Abcam #ab58713, 1:500), HA (Santa Cruz Biotechnology #sc-7392, 1:500), and γ-tubulin (Santa Cruz Biotechnology #sc-17787, 1:500). .. After binding with primary antibodies, membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies, including anti-mouse IgG (Bio-Rad #BR170-6516, 1:3000, Hercules, CA, USA), anti-rabbit IgG (Bio-Rad #BR170-6515, 1:3000), and anti-goat IgG (Thermo Fisher Scientific #31400, 1:10,000), for 1 h. Protein bands were visualized using an enhanced chemiluminescence kit (Advansta #K-12043-D20) and imaged on a ChemiDoc XRS system (Bio-Rad). ..

    Article Title: Hepatitis C Virus Core Induces p53 Ser-15 Phosphorylation to Facilitate E6-Associated Protein-Mediated Proteasomal Degradation of p53
    Article Snippet: After blocking, membranes were incubated with primary antibodies, including total p53 (Santa Cruz Biotechnology #sc-126, 1:1000), pSer-15 p53 (Cell Signaling Technology #9284, 1:1000), pSer-20 p53 (Cell Signaling Technology #9285, 1:1000), E6AP (Thermo Fisher Scientific #PA3-843, 1:2000), MDM2 (Santa Cruz Biotechnology #sc-965, 1:1000), pSer-1981 ATM (Abcam #ab5883, 1:1000), pThr-68 Chk2 (Abcam #ab2661, 1:1000), c-Myc (Santa Cruz Biotechnology #sc-40, 1:500), HCV Core (Abcam #ab58713, 1:500), HA (Santa Cruz Biotechnology #sc-7392, 1:500), and γ-tubulin (Santa Cruz Biotechnology #sc-17787, 1:500). .. After binding with primary antibodies, membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies, including anti-mouse IgG (Bio-Rad #BR170-6516, 1:3000, Hercules, CA, USA), anti-rabbit IgG (Bio-Rad #BR170-6515, 1:3000), and anti-goat IgG (Thermo Fisher Scientific #31400, 1:10,000), for 1 h. Protein bands were visualized using an enhanced chemiluminescence kit (Advansta #K-12043-D20) and imaged on a ChemiDoc XRS system (Bio-Rad). ..



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